RESEARCH ON SAMPLE MANAGEMENT AND ANALYSIS
Research on sample management and analysis carried out within BBMRI-LPC (WP9) aims to provide a scientific evidence base for higher performance instrumentation, methodologies and protocols in biobanking. In order to achieve real-life interoperability of services, it is of great importance to carry out research on processes used by service providers (biobanks) and balance them with customer demands on costs, speed, versality and sample quality.
To achieve the goals set for the sample management and analysis research, several large-scale biobanking facilities will be mapped for their processes, instrumentation, quality control, results, costs and speed (particularly DNA and RNA isolation and integrity evaluation methods). Evidence-based processes and protocols will then be launched for joint use and once proven fit-for-purpose in operational testing at the large-scale biobanking facilities, they will be disseminated throughout the LPC network.
In practice, sample management and analysis research will:
- Establish a development and evaluation platform for new processes and protocols
- Promote efficiency and quality in sample collection, processing, delivery and analysis
- Perform validation of the recommended standard protocols
A joint agreement on an evaluation platform for new processes and protocols
It has been increasingly clear that to achieve real-life interoperability of services there is a need to focus on a research program on the processes used by the service providers (biobankers), balanced with customer (researcher) demands on costs, speed, versatility and sample quality. At least four large-scale biobanking facilities have participated to provide an evidence base to underpin continued integration and improvement of biobanking facilities. Services provided at the collaborating centers are being mapped in terms of processes, instrumentation, QC measures and results, cost and speed. Data accumulated will be used to launch and test evidence-based protocols for joint use at the participating core facilities, including high quality DNA and RNA isolation and integrity evaluation methods. Once proven by the participating facilities, processes and protocols will be disseminated throughout the LPC network.
The increasing level of interoperability of the participating centers has been used to create a large-scale development and evaluation platform for evaluation of new processes and protocols, developed within LPC or elsewhere. Here we present the first outline of such an evaluation platform (Deliverable 9.1).
When required, validation of the recommended standard protocols will be performed during the ongoing studies both from a process perspective and in terms of quality and robustness of the results obtained in the new high throughput analyses.
Biospecimen Reporting Improving Sample Quality (BRISQ)
Based on consecutive meetings/workshops with the partners directly involved in WP9 in addition to several other biobanks within BBMRI-LPC (25 in total), a recommended standardization for sample handling, based on a Biospecimen Reporting Improving Sample Quality (BRISQ) report has been developed.
It was decided to use the web based MolMeth database for building a BRISQ-table. BBMRI.se is serving as the coordinator for compiling information from participating biobanks into MolMeth (http://www.molmeth.org/). A comprehensive sample handling protocol has been developed for serum, plasma, urine, whole blood, RNA, DNA, based on contributions from 25 European biobanks participating in the a network for biobank technologies.
This biobank-specific BRISQ is designed as a dynamic document where new information, protocols/SOPS, amendments etc can be added or revised according to new requirements and knowledge.